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TitleMercaptobenzothiazole-on-Gold Biosensor Systems for Organophosphate and Carbamate Pesticide Compounds.
AuthorSomerse, Vernon Sydwill.
Subject Polyaniline
Subject Acetylcholinesterase
Subject Self-Assembled Monolayer
Subject Organophosphates
Subject Carbamates
Subject Pesticides
Subject Organic Phase Amperometric Biosensor
Subject Nanomolar Detection Limit
Subject Voltammetry.
TypeThesis and dissertation
AbstractThis study firstly reports the development, characterisation, and application of thick-film acetylcholinesterase (AChE) biosensors based on a gold electrode modified with a mercaptobenzothiazole (MBT) self-assembled monolayer and either poly(omethoxyaniline) (POMA) or poly(2,5-dimethoxyaniline) (PDMA) in the presence of polystyrene(4-sulphonic acid) (PSSA). The Au/MBT/POMA-PSSA/AChE and Au/MBT/PDMA-PSSA/AChE biosensors were then applied to successfully detect standard organophosphorous and carbamate pesticides in a 0.1 M phosphate buffer, 0.1 M KCl (pH 7.2) solution. Secondly, it reports the construction of the Au/MBT/PANI/AChE/PVAc thick-film biosensor for the determination of certain organophosphate and carbamate pesticide solutions in selected aqueous organic solvent solutions.